Asabe K, Tsuji K, Handa N, Kajiwara M, Suita S
Department of Pediatric Surgery, Oita Prefectural Hospital, Oita, Japan.
Pediatr Surg Int. 1998 Nov;14(1-2):36-9. doi: 10.1007/s003830050430.
Clara cell 10 kDa protein (CC10) has been thought to be fairly specific to Clara cells and a major secretory protein that is both synthesized and released from Clara cells. In the present study, morphometric analyses of the immunohistochemical expression of CC10 were carried out on the bronchioles of human neonates with congenital diaphragmatic hernia (CDH) and then compared with morphometric analyses from a gestationally and postnatally age-matched control group in order to clarify the immaturity of Clara cells in CDH lungs. No difference was found in CC10 expression between the affected side and the unaffected side of the lungs in the CDH group. However, compared with the lungs of the control group, the CDH group showed a significant decrease in CC10 expression, namely, the ratio of CC10-positive cells per bronchiole, per unit perimeter of bronchiole, and per unit bronchiolar surface area. These results suggest that in the lungs of CDH cases, a possible delay in either functional maturation or the development of CC10 synthesis by the bronchioles may exist, and this retardation of functional maturation of the airway is also considered to play a role in the postnatal respiratory insufficiency observed in CDH patients.
克拉拉细胞10 kDa蛋白（CC10）一直被认为是克拉拉细胞相当特异的一种主要分泌蛋白，由克拉拉细胞合成并释放。在本研究中，对患有先天性膈疝（CDH）的人类新生儿细支气管进行了CC10免疫组化表达的形态计量学分析，然后与来自孕周和出生后年龄匹配的对照组的形态计量学分析进行比较，以阐明CDH肺中克拉拉细胞的不成熟情况。在CDH组中，肺的患侧和未患侧之间CC10表达未发现差异。然而，与对照组的肺相比，CDH组的CC10表达显著降低，即每根细支气管、每单位细支气管周长和每单位细支气管表面积中CC10阳性细胞的比例。这些结果表明，在CDH病例的肺中，可能存在细支气管功能成熟或CC10合成发育的延迟，并且这种气道功能成熟的延迟也被认为在CDH患者出生后观察到的呼吸功能不全中起作用。
