Kawamura M T, Carvalho M G
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Arch Insect Biochem Physiol. 1998;39(2):47-54. doi: 10.1002/(SICI)1520-6327(1998)39:2<47::AID-ARCH1>3.0.CO;2-5.
The influence of extracellular calcium concentration on mosquito cells was investigated in Aedes albopictus cells cultured in a medium with different amounts of calcium. Protein synthesis in cells incubated in low calcium culture medium was inhibited when compared to control cells. This inhibition was reversed by addition of calcium to the culture medium. Two calcium-induced proteins of approximately 70,000 and 80,000 daltons were detected when calcium was added to the extracellular medium of cells incubated in low calcium medium for longer than 2 h. Northern-blot analysis indicated that Hsp70 (heat shock protein of 70,000 dalton) specific mRNA is present in cells that were cultured in low calcium medium suggesting that the 70,000 dalton protein is a member of the Hsp70 family. Our results indicate that extracellular calcium concentration can modify the gene expression pattern in A. albopictus cells and the absence of calcium in the culture medium could be considered a stress factor.
在含有不同钙含量的培养基中培养的白纹伊蚊细胞中，研究了细胞外钙浓度对蚊子细胞的影响。与对照细胞相比，在低钙培养基中孵育的细胞中的蛋白质合成受到抑制。向培养基中添加钙可逆转这种抑制作用。当向在低钙培养基中孵育超过2小时的细胞的细胞外培养基中添加钙时，检测到两种钙诱导的蛋白质，分子量约为70,000和80,000道尔顿。Northern印迹分析表明，在低钙培养基中培养的细胞中存在Hsp70（70,000道尔顿的热休克蛋白）特异性mRNA，这表明70,000道尔顿的蛋白质是Hsp70家族的成员。我们的结果表明，细胞外钙浓度可以改变白纹伊蚊细胞中的基因表达模式，培养基中钙的缺乏可被视为一种应激因素。
