Noia G, Romano D, De Santis M, Gozzo M L, Colacicco L, Mariorenzi S, Straface G, Rumi C, Caruso A, Mancuso S
Department of Obstetrics and Gynecology, Department of Chemistry and Clinical Chemistry, Department of Hematology, Catholic University of Sacred Heart, Largo A. Gemelli n. 8, Rome, 00168, Italy.
Clin Immunol. 1999 Jan;90(1):115-8. doi: 10.1006/clim.1998.4614.
This study aims at observing and comparing the antigen expression of some fetal T- and B-lymphocyte subpopulations in Rh-isoimmunization, which determines anemic hypoxia in the fetus, and nonimmune fetal hydrops (NIFH) which, even if there are some etiological factors involved, causes hipoxic hypoxia in the fetus. Twelve fetuses were studied by way of 30 fetal blood samples obtained by ultrasound-guided cordocentesis between the 20th and 36th gestational week. Twenty-four blood samples in all where taken from the eight fetuses with Rh-isoimmunization. Six blood samples were obtained from the four fetuses with NIFH. The lymphocyte phenotypes studied by monoclonal antibodies and flow cytometry were the following: CD3, CD4, CD8, expression of T-lymphocyte subpopulations; BsIg, CD19, expression of B-lymphocyte subpopulations. We observed a near-normal maturation process in fetuses with Rh isoimmunization, whereas in fetuses with NIFH we observed inhibition and/or delayed expression of T-lymphocytes. An early and increased B-lymphocyte activation marked a cooperation between the two systems in the early gestational periods.
本研究旨在观察和比较某些胎儿T淋巴细胞和B淋巴细胞亚群在Rh血型同种免疫（可导致胎儿贫血性缺氧）和非免疫性胎儿水肿（NIFH，即使涉及一些病因，也会导致胎儿缺氧性缺氧）中的抗原表达。通过超声引导下的脐静脉穿刺术，在妊娠第20至36周期间采集30份胎儿血样，对12例胎儿进行研究。其中，从8例Rh血型同种免疫的胎儿中采集了24份血样，从4例非免疫性胎儿水肿的胎儿中采集了6份血样。通过单克隆抗体和流式细胞术研究的淋巴细胞表型如下：CD3、CD4、CD8，T淋巴细胞亚群的表达；BsIg、CD19，B淋巴细胞亚群的表达。我们观察到Rh血型同种免疫的胎儿成熟过程接近正常，而在非免疫性胎儿水肿的胎儿中，我们观察到T淋巴细胞表达受到抑制和/或延迟。早期且增强的B淋巴细胞活化表明在妊娠早期两个系统之间存在协同作用。
