Beste K W, Duchanova H, Hellige G, Prennschütz-Schützenau H, Vennebusch H, Bretschneider H J
Pflugers Arch. 1976 Sep 30;365(2-3):249-55. doi: 10.1007/BF01067025.
With a newly developed quadrupole mass spectrometer several blood gas partial pressures are recorded simultaneously and continuously. In the mass filter the separation of the ions to be detected takes place in a hyperbolic electric field with variable direct and alternating voltages with a constant frequency of 2.4 MHz. For blood gas analysis a fully relaxed experimental animal was artifically ventilated and provided with arterial and venous catheters. Each catheter consisted flexible steel tubing (external diameter 0.6 mm) slotted near the end and covered with a silicone rubber membrane. The distance from the measuring tip to the mass spectrometer was 150 cm. Experiments with blood, in vivo equilibrated with gas mixtures, showed good reproducibility; the mean error of the helium partial pressure, for example, was less than 4%. During respiration using a gas mixture with 40% argon, the time constant t63% was found to be 55 s for the whole experimental arrangement including the experimental animal.
使用新开发的四极杆质谱仪可以同时连续记录几种血气分压。在质量过滤器中，待检测离子的分离在具有可变直流和交流电压的双曲线电场中进行，恒定频率为2.4MHz。对于血气分析，将一只完全放松的实验动物进行人工通气，并插入动脉和静脉导管。每个导管由末端开槽并覆盖有硅橡胶膜的柔性钢管（外径0.6mm）组成。从测量尖端到质谱仪的距离为150cm。用与气体混合物进行体内平衡的血液进行的实验显示出良好的重现性；例如，氦分压的平均误差小于4%。在使用含40%氩气的气体混合物进行呼吸时，发现整个实验装置（包括实验动物）的时间常数t63%为55s。
