Di Genaro M S, Escudero M E, Muñoz E, Aguilera C, Scardapane L, de Guzmán A M
Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Argentina.
Microbiol Immunol. 1998;42(11):781-8. doi: 10.1111/j.1348-0421.1998.tb02352.x.
Yersinia enterocolitica is enteropathogenic for humans and rodents. Immune protection from oral and respiratory pathogens may be most effectively elicited following intranasal (i.n.) vaccination. An experimental murine intranasal challenge model was used to evaluate the immunogenicity of a Y. enterocolitica O:8 cellular extract (CE) in mucosa. This antigenic preparation has demonstrated to induce protection by subcutaneous immunization. Mice were immunized intranasally with two doses of CE. Immunized and nonimmunized animals were challenged with 5 x 10(6) colony-forming units (CFU) by nasal infection. Antibodies in serum and bronchoalveolar lavage (b.a.l.) fluid were assessed before and 48 hr after challenge. The CFU were determined by analysis of lung homogenate samples. The CE immunization induced significant b.a.l.-specific IgA and IgG, and serum-specific IgG, IgA and IgM. Histopathological studies 24 and 48 hr postchallenge demonstrated that immunization protected against progressive lesions resulting from Y. enterocolitica invasion of the pulmonary mucosa. The CFU in the lungs showed that CE immunization led to significant clearance as compared to the bacterial level in nonimmunized controls. From the results obtained, it can be concluded that CE can induce local and systemic immunity and protect against nasal infection.
小肠结肠炎耶尔森菌对人类和啮齿动物具有肠道致病性。鼻内（i.n.）接种疫苗后，可能最有效地引发针对口腔和呼吸道病原体的免疫保护。使用实验性小鼠鼻内攻击模型来评估小肠结肠炎耶尔森菌O:8细胞提取物（CE）在黏膜中的免疫原性。这种抗原制剂已证明通过皮下免疫可诱导保护作用。用两剂CE对小鼠进行鼻内免疫。对免疫和未免疫的动物通过鼻内感染用5×10⁶菌落形成单位（CFU）进行攻击。在攻击前和攻击后48小时评估血清和支气管肺泡灌洗（b.a.l.）液中的抗体。通过分析肺匀浆样本确定CFU。CE免疫诱导了显著的b.a.l.特异性IgA和IgG以及血清特异性IgG、IgA和IgM。攻击后24小时和48小时的组织病理学研究表明，免疫可防止小肠结肠炎耶尔森菌侵袭肺黏膜导致的进行性病变。肺中的CFU表明，与未免疫对照的细菌水平相比，CE免疫导致细菌显著清除。从获得的结果可以得出结论，CE可诱导局部和全身免疫并预防鼻内感染。
