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[异源转化:探寻利用纳豆芽孢杆菌DNA实现枯草芽孢杆菌高效转化的一种解释]

[Heterologous transformation: search for an explantation of the high effectiveness of Bacillus subtilis transformation achieved by using Bacillus natto DNA].

作者信息

Cherepenko E I, Tikhonova T N

出版信息

Tsitol Genet. 1978 Mar-Apr;12(2):10914.

PMID:98872
Abstract

A higher efficiency of B. subtilis cells transformation by B. natto DNA is true for every concentration of DNA and is not due to the helping effect known for streptococci, strain Challis. In competition experiments for DNA uptake unlabelled B. natto and B. subtilis DNAs when added to transforming H3-DNA reduced the radioactivity of the samples by a factor of three and two, respectively. A conclusion is drawn that a higher rate of B. natto DNA penetration to the B. subtilis cells results in a higher efficiency of their transformation.

摘要

对于每一种DNA浓度,纳豆芽孢杆菌DNA对枯草芽孢杆菌细胞的转化效率更高,这并非由于已知的链球菌Challis菌株的辅助作用。在DNA摄取的竞争实验中,未标记的纳豆芽孢杆菌和枯草芽孢杆菌DNA添加到转化的H3-DNA中时,分别使样品的放射性降低了三分之一和二分之一。得出的结论是,纳豆芽孢杆菌DNA进入枯草芽孢杆菌细胞的速率更高,导致其转化效率更高。

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