Hong M
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts, 01003,
J Magn Reson. 1999 Jan;136(1):86-91. doi: 10.1006/jmre.1998.1631.
A technique for obtaining dipolar-mediated INADEQUATE NMR spectra with a large spectral window in the double-quantum dimension is presented. Using the dipolar recoupling sequence C7 to excite the double-quantum coherence under magic-angle spinning, the technique involves incrementing the evolution period in synchrony with the phase of the radiofrequency pulses in the C7 sequence. The technique is demonstrated on a uniformly 13C-labeled amino acid and an extensively 13C-labeled protein to identify 13C connectivity patterns for spectral assignment.
本文提出了一种在双量子维度中获得具有大光谱窗口的偶极介导的INADEQUATE NMR光谱的技术。利用偶极再耦合序列C7在魔角旋转下激发双量子相干，该技术包括与C7序列中射频脉冲的相位同步增加演化周期。该技术在均匀13C标记的氨基酸和广泛13C标记的蛋白质上进行了演示，以识别用于光谱归属的13C连接模式。
