单克隆抗体ONS-M21可识别神经胶质瘤和髓母细胞瘤中的整合素α3。
Monoclonal antibody ONS-M21 recognizes integrin alpha3 in gliomas and medulloblastomas.
作者信息
Kishima H, Shimizu K, Tamura K, Miyao Y, Mabuchi E, Tominaga E, Matsuzaki J, Hayakawa T
机构信息
Department of Neurosurgery, Osaka University Medical School, Suita, Japan.
出版信息
Br J Cancer. 1999 Jan;79(2):333-9. doi: 10.1038/sj.bjc.6690053.
The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin alpha3. When the integrin alpha3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin alpha3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin alpha3, might be useful in the diagnosis of gliomas and medulloblastomas.
单克隆抗体ONS-M21可识别神经胶质瘤和髓母细胞瘤细胞表面发现的一种抗原，但不与正常脑组织的抗原发生反应。我们通过抗体结合亲和柱纯化并鉴定了140-kDa蛋白。这种140-kDa抗原具有与整联蛋白α3的氨基末端区域和内部结构域的五个部分同源的序列。当扩增整联蛋白α3相关序列并用于分析神经胶质瘤和髓母细胞瘤手术标本的mRNA时，整联蛋白α3 mRNA的转录水平似乎与恶性程度呈定量相关。这些发现表明，与整联蛋白α3反应的ONS-M21抗体可能有助于神经胶质瘤和髓母细胞瘤的诊断。
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