Tim9，线粒体中TIM22.54转位酶的一个新组分。
Tim9, a new component of the TIM22.54 translocase in mitochondria.
作者信息
Adam A, Endres M, Sirrenberg C, Lottspeich F, Neupert W, Brunner M
机构信息
Institut für Physiologische Chemie der Universität München, Goethestrasse 33, 80336 München, Germany.
出版信息
EMBO J. 1999 Jan 15;18(2):313-9. doi: 10.1093/emboj/18.2.313.
Abstract
We have identified Tim9, a new component of the TIM22.54 import machinery, which mediates transport of proteins into the inner membrane of mitochondria. Tim9, an essential protein of Saccharomyces cerevisiae, shares sequence similarity with Tim10 and Tim12. Tim9 is located in the mitochondrial intermembrane space and is organized into two distinct hetero-oligomeric assemblies with Tim10 and Tim12. One complex contains Tim9 and Tim10. The other complex contains Tim9, Tim10 and Tim12 and is tightly associated with Tim22 in the inner membrane. The TIM9.10 complex is more abundant than the TIM9.10.12 complex and mediates partial translocation of mitochondrial carriers proteins across the outer membrane. The TIM9.10.12 complex assists further translocation into the inner membrane in association with TIM22.54.
摘要
我们鉴定出Tim9，它是TIM22.54导入机制的一个新组分，介导蛋白质转运至线粒体内膜。Tim9是酿酒酵母的一种必需蛋白，与Tim10和Tim12具有序列相似性。Tim9位于线粒体膜间隙，与Tim10和Tim12组成两种不同的异源寡聚体组合。一种复合物包含Tim9和Tim10。另一种复合物包含Tim9、Tim10和Tim12，并在内膜中与Tim22紧密结合。TIM9.10复合物比TIM9.10.12复合物更丰富，介导线粒体载体蛋白跨外膜的部分转运。TIM9.10.12复合物与TIM22.54协同作用，协助进一步转运至内膜。
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