Pompei P, Severini R, Costa G, Massi M, Fattoretti P, Bertoni-Freddari C
Department of Pharmacological Sciences and Experimental Medicine, University of Camerino, Via Scalzino 3, 62032 Camerino (MC), Italy.
Brain Res Mol Brain Res. 1999 Jan 22;64(1):132-6. doi: 10.1016/s0169-328x(98)00317-9.
The present study examined the effects of ageing on preprotachykinin-A (PPT-A) mRNA levels in discrete regions of the rat brain. Semiquantitative analysis of silver grains revealed a 16% statistically significant decrease in PPT-A mRNA in the shell of the nucleus accumbens (AcbSh), a 27.6% statistically significant lower level of PPT-A mRNA in the olfactory tubercle (Tu), a 19.2% and 31. 5% statistically significant decrease in PPT-A mRNA in the dorsal and ventral caudate-putamen (d-CPu) (v-CPu), respectively, a 30% statistically significant lower expression of PPT-A mRNA in the bed nucleus of the stria terminalis (BNST), a 33.7% statistically significant decrease in PPT-A mRNA in the habenula (Hb) and a 30% statistically significant decrease of PPT-A mRNA levels in the postero-dorsal part of the medial amygdala (MePD). No changes in PPT-A mRNA levels were found in the nucleus accumbens, core (AcbC), in the islands of Calleja (Icj), and in the medial preoptic area (mPOA). These results show that ageing of the central nervous system (CNS) is associated with widespread changes in tachykinin gene expression, suggesting that alteration in the tachykinergic system may have implications in the physio-pathology of the elderly.
本研究检测了衰老对大鼠脑离散区域中前速激肽原A（PPT-A）mRNA水平的影响。银颗粒的半定量分析显示，伏隔核壳（AcbSh）中PPT-A mRNA水平有16%的统计学显著下降，嗅结节（Tu）中PPT-A mRNA水平有27.6%的统计学显著降低，背侧和腹侧尾状核-壳核（d-CPu）（v-CPu）中PPT-A mRNA水平分别有19.2%和31.5%的统计学显著下降，终纹床核（BNST）中PPT-A mRNA表达有30%的统计学显著降低，缰核（Hb）中PPT-A mRNA水平有33.7%的统计学显著下降，内侧杏仁核后背部（MePD）中PPT-A mRNA水平有30%的统计学显著下降。在伏隔核核心（AcbC）、Calleja岛（Icj）和内侧视前区（mPOA）中未发现PPT-A mRNA水平有变化。这些结果表明，中枢神经系统（CNS）的衰老与速激肽基因表达的广泛变化有关，提示速激肽能系统的改变可能对老年人的生理病理学有影响。
