Fuks B, Homblé F
Laboratoire de Physiologie Végétale, Campus Plaine, C.P. 206/2, Université Libre de Bruxelles, B-1050, Brussels, Belgium.
Biochim Biophys Acta. 1999 Jan 12;1416(1-2):361-9. doi: 10.1016/s0005-2736(98)00237-5.
It has been shown that chloride channels are located in the envelope membranes of chloroplasts [5,11]. In this report, we use the light-scattering technique to measure quantitatively the rate of anion transport through the inner envelope membrane of isolated intact chloroplasts. Our results permit to assign the anion transport to the inner envelope of chloroplasts. The anionic selectivity determined from the kinetics of light scattering indicates that the chloride pathway is also highly permeable for NO-2 and NO-3. The sulfate and phosphate anions are impermeant. The chloride flux is not inhibited by DIDS or NEM and is temperature-dependent. The activation energy of the transport process suggests that the Cl- flux occurs through a channel.
已经表明，氯离子通道位于叶绿体的包膜膜中[5,11]。在本报告中，我们使用光散射技术定量测量阴离子通过分离的完整叶绿体的内膜的运输速率。我们的结果允许将阴离子运输归因于叶绿体的内膜。从光散射动力学确定的阴离子选择性表明，氯离子途径对NO-2和NO-3也具有高度渗透性。硫酸根和磷酸根阴离子是不可渗透的。氯离子通量不受DIDS或NEM的抑制，并且与温度有关。运输过程的活化能表明Cl-通量是通过通道发生的。
