Fujiwara S, Takagi M, Imanaka T
Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.
Biotechnol Annu Rev. 1998;4:259-84. doi: 10.1016/s1387-2656(08)70073-5.
Archaea is the third domain which is phylogenetically differentiated from the other two domains, bacteria and eucarya. Hyperthermophile within the archaea domain has evolved most slowly retaining many ancestral features of higher eukaryotes. Pyrococcus kodakaraensis KOD1, which grows at 95 degrees C optimally, is a newly isolated hyperthermophilc archaeon. The KOD1 strain possesses a circular genome, whose size is estimated to be approximately 2,036 kb. KOD1 enzymes involved in the genetic information processing system, such as DNA polymerase, Rec protein, aspartyl tRNA synthetase and molecular chaperonin, share features of eukaryotic enzymes. Rapid and accurate PCR method by KOD1 DNA polymerase and enzyme stabilization system by KOD1 chaperonin are also introduced in this article.
古菌是在系统发育上与其他两个域（细菌和真核生物）不同的第三个域。古菌域内的嗜热菌进化最为缓慢，保留了许多高等真核生物的祖先特征。最适生长温度为95摄氏度的柯达嗜热栖热菌KOD1是一种新分离的嗜热古菌。KOD1菌株拥有一个环状基因组，其大小估计约为2036 kb。参与遗传信息处理系统的KOD1酶，如DNA聚合酶、Rec蛋白、天冬氨酰tRNA合成酶和分子伴侣蛋白，具有真核酶的特征。本文还介绍了KOD1 DNA聚合酶的快速准确PCR方法以及KOD1伴侣蛋白的酶稳定系统。
