氨基甲酸酯类杀虫剂兰纳特对骆驼视网膜乙酰胆碱酯酶抑制作用的动力学常数。

Kinetic constants for the inhibition of camel retinal acetylcholinesterase by the carbamate insecticide lannate.

作者信息

Kamal M A, Al-jafari A A

机构信息

Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.

出版信息

J Biochem Mol Toxicol. 1999;13(1):41-6. doi: 10.1002/(sici)1099-0461(1999)13:1<41::aid-jbt5>3.0.co;2-f.

DOI:10.1002/(sici)1099-0461(1999)13:1<41::aid-jbt5>3.0.co;2-f

PMID:9890446

Abstract

We have designed this study to determine various kinetic parameters of camel retinal membrane-bound acetylcholinesterase (AChE; EC 3.1.1.7) inhibition by carbamate insecticide lannate [methyl N-[[(methylamino)carbonyl]oxy] ethanimidothioate]. All these kinetic constants were derived by simple graphical methods. The value of kinetic parameters was estimated as follows: 0.061 microM)(-1), 1.14 (microM)(-1), 0.216 microM, 0.016 min(-1), 0.0741 (gammaM min)(-1) 0.746 microM, and 4.42 microM for velocity constant (Kv), new inhibition constant (Knic), dissociation constant (Kd), carbamylation rate constant (k2c), overall carbamylation rate constant (k'2), 50% inhibition constant (K150), and 99% inhibition constant (K199), respectively. These unique methods may be used to estimate such kinetic parameters for time-dependent inhibition of enzymes by variety of chemicals, insecticides, herbicides, and drugs.

摘要

我们设计了这项研究，以确定氨基甲酸酯类杀虫剂兰纳特[甲基N-[[(甲氨基)羰基]氧基]乙亚胺硫酯]对骆驼视网膜膜结合乙酰胆碱酯酶（AChE；EC 3.1.1.7）抑制作用的各种动力学参数。所有这些动力学常数均通过简单的图形方法得出。动力学参数的值估计如下：速度常数（Kv）为0.061微摩尔⁻¹、新抑制常数（Knic）为1.14微摩尔⁻¹、解离常数（Kd）为0.216微摩尔、氨甲酰化速率常数（k2c）为0.016分钟⁻¹、总氨甲酰化速率常数（k'2）为0.0741（伽马摩尔·分钟⁻¹）、50%抑制常数（K150）为0.746微摩尔，以及99%抑制常数（K199）为4.42微摩尔。这些独特的方法可用于估计各种化学物质、杀虫剂、除草剂和药物对酶的时间依赖性抑制作用的此类动力学参数。