基于电子显微镜、荧光分析法和电取向光谱法对MTPN11分枝杆菌噬菌体与宿主细胞相互作用的研究
[Study of the interaction of MTPN11 mycobacteriophage with host cells based on electron microscopy, fluorimetry, and electro-oriented spectroscopy].
作者信息
Zhilenkov E L, Shemiakin I G, Fomchenkov V M, Ivanov A Iu, Gavriushkin A V, Oborotov M V
机构信息
State Research Center for Applied Microbiology, Obolensk, Russia.
出版信息
Mikrobiologiia. 1998 Sep-Oct;67(5):666-71.
According to electron-microscopic data, various cells in the M. smegmatis ATCC607 population interact differently with phage MTPH11. Fluorometric studies of phage-host interactions were performed using a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfonate (ANS). Changes in the electric characteristics of mycobacterial cells infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spectroscopy for rapid phage typing of mycobacteria is discussed.
根据电子显微镜数据，耻垢分枝杆菌ATCC607群体中的各种细胞与噬菌体MTPH11的相互作用方式不同。使用膜亲和荧光探针8-苯胺基-1-萘磺酸盐（ANS）对噬菌体-宿主相互作用进行了荧光测定研究。通过电取向（EO）光谱研究了感染噬菌体的分枝杆菌细胞的电学特性变化。讨论了使用荧光测定法和EO光谱对分枝杆菌进行快速噬菌体分型的问题。
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