Kozlova A V, Omel'ianchuk L V
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Ontogenez. 1998 Sep-Oct;29(5):366-72.
Transposition of the P[1ArB] vector was used to generate three mutations inducing sterility in homozygous males: ms(3)P50, ms(3)P122, and ms(3)P115 located within 67A4-B13, 92A2-14, and 75D regions on the polytene map. Staining for beta-galactosidase synthesized by P[1ArB] allowed us to study enhancer activity of these ms genes in the testicles. Their activity correlated to the presence of the YS arm but was little affected by the presence of YL arm of Y chromosome.
利用P[1ArB]载体的转座作用产生了三个在纯合雄性中诱导不育的突变:ms(3)P50、ms(3)P122和ms(3)P115,它们位于多线染色体图谱上的67A4-B13、92A2-14和75D区域。对由P[1ArB]合成的β-半乳糖苷酶进行染色,使我们能够研究这些ms基因在睾丸中的增强子活性。它们的活性与YS臂的存在相关,但受Y染色体YL臂存在的影响较小。