Kuriyama S, Yamazaki M, Mitoro A, Tsujimoto T, Kikukawa M, Okuda H, Tsujinoue H, Nakatani T, Yoshiji H, Toyokawa Y, Nagao S, Fukui H
Third Department of Internal Medicine, Nara Medical University, Japan.
Anticancer Res. 1998 Nov-Dec;18(6A):4181-8.
Despite intensive efforts in the treatment of hepatocellular carcinoma (HCC), its prognosis remains poor, mainly because of intrahepatic metastasis. It is, therefore, important to investigate the invasive and metastatic behavior of HCC. To examine this, murine HCC cells were labeled with the fluorescent carbocyanine dye, DiI and implanted under the capsule of the liver of syngeneic mice. Optimal conditions are described for labeling HCC cells with DiI. Histological analysis using fluorescent and confocal microscopes revealed that HCC cells migrate to and invade the adjacent portal vein, but not the adjacent central vein. Conversely, DiI-labeled hepatocytes were shown not to migrate in the liver. These results suggest that intrahepatic metastasis of HCC occurs by invading the portal venous system. Furthermore, it is indicated that orthotopic implantation of fluorescent dye-labeled tumor cells may be a convenient and useful method to investigate the invasive and metastatic behavior of various types of cancer.
尽管在肝细胞癌（HCC）的治疗方面付出了巨大努力，但其预后仍然很差，主要原因是肝内转移。因此，研究HCC的侵袭和转移行为很重要。为了对此进行研究，将小鼠HCC细胞用荧光碳氰染料DiI标记，并植入同基因小鼠的肝包膜下。描述了用DiI标记HCC细胞的最佳条件。使用荧光显微镜和共聚焦显微镜进行的组织学分析显示，HCC细胞迁移并侵入相邻的门静脉，但不侵入相邻的中央静脉。相反，DiI标记的肝细胞在肝脏中未显示出迁移。这些结果表明，HCC的肝内转移是通过侵入门静脉系统发生的。此外，表明荧光染料标记的肿瘤细胞原位植入可能是一种方便且有用的方法，用于研究各种类型癌症的侵袭和转移行为。
