Sakagami H, Satoh M, Yokote Y, Takano H, Takahama M, Kochi M, Akahane K
Department of Dental Pharmacology, Meikai University School of Dentistry, Saitama, Japan.
Anticancer Res. 1998 Nov-Dec;18(6A):4303-6.
The amino acid utilization between human promyelocytic leukemia (HL-60), human oral squamous carcinoma (HSC-2, HSC-4, NA), human salivary gland tumor (HSG) and rat neuron cells (PC-12) were compared, using amino acid analyzer. All these cells consumed four essential amino acids (valine, methionine, isoleucine, leucine), glutamine and arginine, whereas they produced glycine, alanine and ammonia, without significantly affecting threonine, tyrosine, phenylalanine, histidine or lysine concentration. Serine and glutamine utilization varied considerably from cell to cell. HL-60 cells consumed serine and arginine at much higher rates than other cells. Serine depletion accumulated the G1 arrested cells, and produced increasing numbers of the apoptotic cells. Supplementation of serine significantly extended the period of logarithmic cell growth. During apoptosis induction of HL-60 cells by dopamine, sodium ascorbate or sodium 5,6-benzylidene-L-ascorbate, the oxidation of methionine to methionine sulfoxide was enhanced, but the consumption of serine, glutamine and arginine was reduced. In the presence of HL-60 cells, the methionine oxidation was significantly inhibited, suggesting the antioxidant action of the cells. These present study suggests the importance of re-evaluation of culture condition for each cell lines.
使用氨基酸分析仪比较了人早幼粒细胞白血病（HL - 60）、人口腔鳞状细胞癌（HSC - 2、HSC - 4、NA）、人唾液腺肿瘤（HSG）和大鼠神经细胞（PC - 12）之间的氨基酸利用情况。所有这些细胞都消耗四种必需氨基酸（缬氨酸、蛋氨酸、异亮氨酸、亮氨酸）、谷氨酰胺和精氨酸，同时产生甘氨酸、丙氨酸和氨，而对苏氨酸、酪氨酸、苯丙氨酸、组氨酸或赖氨酸的浓度没有显著影响。丝氨酸和谷氨酰胺的利用在不同细胞间差异很大。HL - 60细胞消耗丝氨酸和精氨酸的速率比其他细胞高得多。丝氨酸耗竭会使处于G1期停滞的细胞积累，并产生越来越多的凋亡细胞。补充丝氨酸可显著延长细胞对数生长期。在多巴胺、抗坏血酸钠或5,6 - 亚苄基 - L - 抗坏血酸钠诱导HL - 60细胞凋亡的过程中，蛋氨酸氧化为蛋氨酸亚砜的过程增强，但丝氨酸、谷氨酰胺和精氨酸的消耗减少。在有HL - 60细胞存在的情况下，蛋氨酸氧化受到显著抑制，表明细胞具有抗氧化作用。这些研究表明重新评估每种细胞系的培养条件具有重要意义。
