使用C30键合相通过反相高效液相色谱法鉴定牛视网膜中的叶黄素和玉米黄质立体异构体。

Reversed-phase high-performance liquid chromatographic identification of lutein and zeaxanthin stereoisomers in bovine retina using a C30 bonded phase.

作者信息

Dachtler M, Kohler K, Albert K

机构信息

University of Tübingen, Institute of Organic Chemistry, Germany.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):211-6. doi: 10.1016/s0378-4347(98)00431-9.

DOI:10.1016/s0378-4347(98)00431-9

PMID:9892084

Abstract

An efficient reversed-phase high-performance liquid chromatographic assay with ultraviolet detection at 450 nm for simultaneous determination of lutein and zeaxanthin stereoisomers in bovine retina is described. The procedure involves rapid and careful one-step hexane extraction of the carotenoids from the homogenized liquid and enrichment by on-line solid phase extraction on a polystyrene cartridge. The substances were eluted at a flow rate of 1 ml/min with acetone-water (85:15, v/v) on a C30 reversed-phase column. C30 phases exhibit superior shape selectivity for the separation of carotenoid stereoisomers compared with conventional C18 phases. For comparison a mixture of iodine-isomerized lutein and zeaxanthin standards was separated.

摘要

描述了一种高效反相高效液相色谱法，该方法采用450nm紫外检测，用于同时测定牛视网膜中叶黄素和玉米黄质立体异构体。该方法包括从匀浆中快速、小心地一步己烷提取类胡萝卜素，并通过在聚苯乙烯柱上进行在线固相萃取进行富集。在C30反相柱上，以1ml/min的流速用丙酮-水（85:15，v/v）洗脱这些物质。与传统的C18相相比，C30相对类胡萝卜素立体异构体的分离表现出优异的形状选择性。为作比较，分离了碘异构化叶黄素和玉米黄质标准品的混合物。