使用四根串联柱的高效液相色谱法同时测定5-氟尿嘧啶和尿嘧啶。
Simultaneous determination of 5-fluorouracil and uracil by high-performance liquid chromatography using four serial columns.
作者信息
House L K, Ramírez J, Ratain M J
机构信息
Department of Medicine, The University of Chicago, IL 60637, USA.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):245-50. doi: 10.1016/s0378-4347(98)00424-1.
A sensitive assay was developed for the quantitation of 5-fluorouracil (5-FU) and uracil using liquid-liquid extraction (LLE) and HPLC with UV detection. Analyses were performed with four microBondapak C18 columns connected in series using 20 mM acetic acid with 1% ACN as mobile phase. The calibration curves were linear across the range of 26-1000 ng ml(-1) (0.21-7.8 microM) for 5-FU and 1.0-14.0 microg ml(-1) (0.01-110 microM) for uracil. This assay has been implemented to determine the plasma concentrations for pharmacokinetic studies for 5-FU and uracil in conjunction with clinical trials.
开发了一种灵敏的测定方法，用于使用液-液萃取（LLE）和带紫外检测的高效液相色谱法（HPLC）对5-氟尿嘧啶（5-FU）和尿嘧啶进行定量。使用串联的四根微Bondapak C18柱进行分析，流动相为含1%乙腈的20 mM乙酸。5-FU的校准曲线在26 - 1000 ng ml⁻¹（0.21 - 7.8 μM）范围内呈线性，尿嘧啶的校准曲线在1.0 - 14.0 μg ml⁻¹（0.01 - 110 μM）范围内呈线性。该测定方法已用于结合临床试验测定5-FU和尿嘧啶药代动力学研究的血浆浓度。
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