Manaresi E, Gallinella G, Zerbini M, Venturoli S, Gentilomi G, Musiani M
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
J Med Virol. 1999 Feb;57(2):174-8.
Human B19 parvovirus recombinant capsid proteins VP1 and VP2 were expressed in E. coli and purified. Recombinant proteins were used to detect a specific IgG immune response against VP1 and VP2 linear epitopes by immunoblot assay. A total of 222 serum samples from 218 apparently immunocompetent subjects with different clinical conditions and laboratory evaluations with regards to B19 infection were analyzed. The sera had previously been tested for B19 DNA and for specific IgM and IgG against VP2 conformational antigens by ELISA assay. The data show that, during the active or very recent phase of infection, IgG anti-VP1 linear epitopes appear in concomitance and with the same frequency as IgG anti-VP2 conformational antigens. IgG against conformational VP2 antigens and against linear VP1 epitopes seem to persist for months or years in the majority of individuals. IgG against VP2 linear epitopes are generally present during the active or very recent phase of infection and during the convalescent phase, while they are present only in about 20% of subjects with signs of a past B19 infection.
人细小病毒B19重组衣壳蛋白VP1和VP2在大肠杆菌中表达并纯化。通过免疫印迹分析,使用重组蛋白检测针对VP1和VP2线性表位的特异性IgG免疫反应。分析了来自218名明显免疫功能正常的受试者的222份血清样本,这些受试者具有不同的临床状况,并针对B19感染进行了实验室评估。这些血清先前已通过ELISA检测B19 DNA以及针对VP2构象抗原的特异性IgM和IgG。数据显示,在感染的活跃期或近期,抗VP1线性表位的IgG与抗VP2构象抗原的IgG同时出现,且频率相同。在大多数个体中,针对构象VP2抗原和线性VP1表位的IgG似乎会持续数月或数年。抗VP2线性表位的IgG通常在感染的活跃期或近期以及恢复期出现,而在仅有过去B19感染迹象的受试者中,只有约20%的人存在这种抗体。