人血清中抗人博卡病毒结构蛋白和非结构蛋白的抗体。
Antibodies against structural and nonstructural proteins of human bocavirus in human sera.
作者信息
Shirkoohi Reza, Endo Rika, Ishiguro Nobuhisa, Teramoto Shinobu, Kikuta Hideaki, Ariga Tadashi
机构信息
Department of Pediatrics, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan.
出版信息
Clin Vaccine Immunol. 2010 Jan;17(1):190-3. doi: 10.1128/CVI.00355-09. Epub 2009 Dec 2.
Abstract
Immunofluorescence assays (IFAs) for detection of human bocavirus (HBoV) proteins (VP1, VP2, NP-1, and NS1) were developed. The VP1 IFA was the most sensitive for detection of IgG antibody and suitable for screening. IgG antibodies in convalescent-phase sera from HBoV-positive patients were detected by VP1 and VP2 IFAs. Sensitivities of NP-1 and NS1 IFAs were low.
摘要
开发了用于检测人博卡病毒（HBoV）蛋白（VP1、VP2、NP-1和NS1）的免疫荧光分析（IFA）。VP1 IFA在检测IgG抗体方面最为灵敏，适用于筛查。通过VP1和VP2 IFA检测了HBoV阳性患者恢复期血清中的IgG抗体。NP-1和NS1 IFA的灵敏度较低。
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