Greenwood-Van Meerveld B, Tyler K, Kuge T, Ogata N
Oklahoma Foundation for Digestive Research, Basic Sciences Laboratory, VA Medical Center, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA.
Aliment Pharmacol Ther. 1999 Jan;13(1):97-102. doi: 10.1046/j.1365-2036.1999.00443.x.
Seirogan is a beechwood extract composed of guaiacol, creosol and other related phenolic compounds which is widely used as an anti-diarrhoeal agent in Asia. Abnormalities in water and electrolyte transport are often the cause of diarrhoea, but the mechanism of action of seirogan on small intestinal and colonic mucosal ion transport is unknown.
To examine the effect of seirogan on electrogenic ion transport in vitro.
Sheets of rat jejunum and colon were mounted in Ussing chambers, and transmural potential difference (PD) was used as an electrical marker of changes in mucosal ion transport. Hypersecretory conditions were induced by acetylcholine (ACh).
Serosal or mucosal application of seirogan (0.1-100 microg/mL) decreased basal jejunal transmural PD. Pre-treatment of the tissue with the neurotoxin, tetrodotoxin, did not inhibit the seirogan-induced changes in basal electrical activity. Seirogan had no effect on basal transmural PD in the ileum and colon. Under ACh-induced hypersecretory conditions in the small intestine and colon, addition of serosal or mucosal seirogan produced antisecretory effects determined indirectly by measurement of transmural PD.
The ability of seirogan to decrease basal transmural PD in the jejunum, and inhibit the ACh-induced electrical responses, may contribute to its anti-diarrhoeal action.
正露丸是一种由愈创木酚、甲酚和其他相关酚类化合物组成的山毛榉木提取物，在亚洲被广泛用作止泻剂。水和电解质转运异常通常是腹泻的原因，但正露丸对小肠和结肠黏膜离子转运的作用机制尚不清楚。
研究正露丸对体外电生性离子转运的影响。
将大鼠空肠和结肠片安装在尤斯灌流小室中，跨膜电位差（PD）用作黏膜离子转运变化的电指标。用乙酰胆碱（ACh）诱导高分泌状态。
浆膜面或黏膜面给予正露丸（0.1 - 100μg/mL）可降低空肠基础跨膜PD。用神经毒素河豚毒素预处理组织，并不抑制正露丸诱导的基础电活动变化。正露丸对回肠和结肠的基础跨膜PD无影响。在小肠和结肠由ACh诱导的高分泌状态下，给予浆膜面或黏膜面正露丸可产生抗分泌作用，通过测量跨膜PD间接确定。
正露丸降低空肠基础跨膜PD以及抑制ACh诱导的电反应的能力，可能有助于其止泻作用。
