Petratos S, Turnbull V J, Papadopoulos R, Ayers M, Gonzales M F
Neuropathology Research Laboratory, Department of Anatomical Pathology, Royal Melbourne Hospital, Victoria, Australia.
Immunol Cell Biol. 1998 Dec;76(6):535-41. doi: 10.1046/j.1440-1711.1998.00778.x.
Plasma samples from 35 individuals with HIV infection but without clinical peripheral neuropathy were screened by ELISA for IgM and IgG antibodies against peripheral myelin. Eighteen of the 35 samples (51%) showed IgM reactivity and 11 (31%) showed IgG reactivity. By comparison, none of 48 samples from healthy blood donors showed IgM or IgG reactivity. Epitopes reacting with these antibodies were identified by TLC immunostaining as sulphatide (GalS) and the gangliosides GM1, GD1a and GD1b. Plasma samples from four people with HIV infection and neuropathy (HIV+PN), six HIV-seronegative individuals with IgM paraproteinaemic demyelinating neuropathy (IgMPDN) and 12 HIV-seronegative individuals with a variety of other neurological disorders (HIV-OND) were also investigated. Two of the four HIV+PN samples showed IgM reactivity with GalS; and two showed IgG reactivity against GalS. Of the six IgMPDN samples, three showed IgM reactivity with GalS. These data indicate that antibodies against peripheral myelin glycolipids, in particular GalS, occur more frequently in HIV infection than in HIV-seronegative individuals with and without neurological disease, and may contribute to subclinical neuropathy in HIV infection.
采用酶联免疫吸附测定法（ELISA）对35例感染HIV但无临床周围神经病变的个体的血浆样本进行检测，以筛查针对周围神经髓磷脂的IgM和IgG抗体。35份样本中有18份（51%）显示IgM反应性，11份（31%）显示IgG反应性。相比之下，48份健康献血者的样本均未显示IgM或IgG反应性。通过薄层层析免疫染色法确定，与这些抗体发生反应的表位为硫脂（GalS）以及神经节苷脂GM1、GD1a和GD1b。还对4例感染HIV且患有神经病变（HIV+PN）的患者、6例IgM副蛋白血症性脱髓鞘性神经病变（IgMPDN）的HIV血清阴性个体以及12例患有各种其他神经系统疾病（HIV-OND）的HIV血清阴性个体的血浆样本进行了研究。4份HIV+PN样本中有2份显示出与GalS的IgM反应性；2份显示出针对GalS的IgG反应性。在6份IgMPDN样本中，3份显示出与GalS的IgM反应性。这些数据表明，针对周围神经髓磷脂糖脂（尤其是GalS）的抗体在HIV感染患者中比在患有和未患有神经系统疾病的HIV血清阴性个体中更频繁出现，并且可能导致HIV感染中的亚临床神经病变。
