Tanada N, Kashimura S, Kageura M, Hara K
Department of Forensic Medicine, Fukuoka University School of Medicine, Japan.
Nihon Hoigaku Zasshi. 1998 Aug;52(4):233-7.
Utility of caffeine analysis for the purpose of forensic hair discrimination was evaluated. Methanol extracts of each 5-cm strand of scalp hair, after its incubation at 37 degrees C for 15 h, was analyzed by gas chromatography-mass spectrometry (GC-MS). For quantitative analysis of caffeine, caffeine-d3 was added as internal standard to the methanol extract. Hair specimens were obtained from three victims and from twenty-two volunteers. The results showed that caffeine levels in hair varied according to each individual; caffeine was detected up to a maximum level of 4.19 ng/5-cm hair. The concentrations of caffeine in hair obtained from four different regions of the scalp in twenty-two volunteers showed a coefficient of variation not greater than 24.7% for each individual. Based on our data, it was concluded that caffeine levels give relatively high reliability for discriminating a victim's hair from others at a criminal or accidental scene, although it should be used in combination with morphological examination and ABO blood group determination.
评估了咖啡因分析用于法医毛发鉴别的效用。每缕5厘米头皮毛发在37摄氏度下孵育15小时后，用甲醇提取，然后通过气相色谱 - 质谱联用仪（GC-MS）进行分析。为了对咖啡因进行定量分析，向甲醇提取物中加入咖啡因 - d3作为内标。毛发样本取自三名受害者和二十二名志愿者。结果表明，毛发中的咖啡因水平因个体而异；检测到的咖啡因最高水平为4.19纳克/5厘米毛发。二十二名志愿者头皮四个不同区域的毛发中咖啡因浓度显示，每个个体的变异系数不大于24.7%。根据我们的数据得出结论，尽管咖啡因水平应与形态学检查和ABO血型测定结合使用，但在刑事或意外现场将受害者的毛发与其他人的毛发区分开来时，咖啡因水平具有相对较高的可靠性。
