晚期结直肠癌中CD44剪接变体表达降低。

Decreased expression of CD44 splicing variants in advanced colorectal carcinomas.

作者信息

Weg-Remers S, Anders M, von Lampe B, Riecken E O, Schüder G, Feifel G, Zeitz M, Stallmach A

机构信息

Department of Internal Medicine II, University of the Saarland, Homburg, Germany.

出版信息

Eur J Cancer. 1998 Sep;34(10):1607-11. doi: 10.1016/s0959-8049(98)00177-4.

DOI:10.1016/s0959-8049(98)00177-4

PMID:9893637

Abstract

CD44v6 expression appears to be associated with adverse prognosis and propensity for metastasis in patients with colorectal cancer. However, expression of CD44 variants in different tumour stages has been poorly characterised. CD44 variant expression was investigated in normal colonic mucosa (n = 36), colorectal adenomas (n = 15), carcinomas (n = 62) and metastases (n = 6) by reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blotting with exon-specific probes. High frequencies of CD44 standard (CD44s) and CD44 epithelial (CD44e) were observed in normal and neoplastic tissue. CD44v2 was seen predominantly in adenomas (27%) and UICCI carcinomas (29%). CD44v5 expression was low in normal mucosa (3%), higher in adenomas and carcinomas (29-33%), independent of tumour stage. CD44v6 expression was low in normal mucosa (6%) and higher in adenomas (47%) and carcinomas (42%). Surprisingly, a significant decrease of CD44v6 was observed in metastatic primary tumours (8%) and metastases (17%) (UICCIV) (P < or = 0.05). Therefore, the concept of CD44v6 conferring metastatic potential to malignant cells cannot be supported by our data.

摘要

CD44v6的表达似乎与结直肠癌患者的不良预后和转移倾向相关。然而，不同肿瘤阶段CD44变体的表达情况尚未得到充分描述。通过逆转录聚合酶链反应（RT-PCR）和使用外显子特异性探针的Southern印迹法，对正常结肠黏膜（n = 36）、大肠腺瘤（n = 15）、癌（n = 62）和转移灶（n = 6）中的CD44变体表达进行了研究。在正常组织和肿瘤组织中均观察到高频率的CD44标准型（CD44s）和CD44上皮型（CD44e）。CD44v2主要见于腺瘤（27%）和UICC I期癌（29%）。CD44v5在正常黏膜中的表达较低（3%），在腺瘤和癌中的表达较高（29 - 33%），且与肿瘤分期无关。CD44v6在正常黏膜中的表达较低（6%），在腺瘤（47%）和癌（42%）中的表达较高。令人惊讶的是，在转移性原发性肿瘤（8%）和转移灶（17%）（UICC IV期）中观察到CD44v6显著降低（P≤0.05）。因此，我们的数据无法支持CD44v6赋予恶性细胞转移潜能这一概念。