Cafaro V, Bracale A, Formiggini F, Notomista E, D'Alessio G, Di Donato A
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Naples, Italy.
Biochimie. 1998 Nov;80(11):905-9. doi: 10.1016/s0300-9084(00)88887-2.
Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric RNases display selective toxicity for malignant cells, which is absent in the monomeric mutants.
天然牛精液核糖核酸酶具有强大的抗肿瘤作用。我们已对无任何细胞毒性作用的单体牛胰核糖核酸酶A进行诱变，以插入二聚体抗肿瘤精液核糖核酸酶亚基相应位置上存在的残基。与天然二聚体精液核糖核酸酶一样，突变二聚体核糖核酸酶对恶性细胞表现出选择性毒性，而单体突变体则没有这种毒性。
