Choi J S, Lee E J, Choi Y H, Jeong Y J, Park J S
Department of Chemistry, Seoul National University, San 56-1, Shillim-dong, Kwanak-ku, Seoul, 151-742, Korea.
Bioconjug Chem. 1999 Jan-Feb;10(1):62-5. doi: 10.1021/bc9800668.
Methoxypoly(ethylene glycol)-block-poly(L-lysine) dendrimer was designed to form a water-soluble complex with plasmid DNA. The copolymer was synthesized by the liquid-phase peptide synthesis method. It was characterized by 1H NMR and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrum. Agarose gel electrophoresis and DNase I protection assay proved that this linear polymer/dendrimer block copolymer assembled spontaneously with plasmid DNA, forming a water-soluble complex which increased the stability of the complexed DNA. Atomic force microscopy of the complex was evaluated at various charge ratios showing that the copolymer/DNA complex was like a globular shape.
甲氧基聚（乙二醇）-嵌段-聚（L-赖氨酸）树枝状聚合物被设计用于与质粒DNA形成水溶性复合物。该共聚物通过液相肽合成法合成。通过1H NMR和基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱对其进行了表征。琼脂糖凝胶电泳和DNase I保护试验证明，这种线性聚合物/树枝状聚合物嵌段共聚物能与质粒DNA自发组装，形成一种水溶性复合物，提高了复合DNA的稳定性。在不同电荷比下对复合物进行了原子力显微镜评估，结果表明共聚物/DNA复合物呈球状。
