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Normalization of RNA hybridization signals by means of SYBR green II-stained 28S or 18S ribosomal RNA and a phosphor imager.

作者信息

Spiess A N, Ivell R

机构信息

Institute for Hormone and Fertility Research, University of Hamburg, Germany.

出版信息

Biotechniques. 1999 Jan;26(1):46-8, 50. doi: 10.2144/99261bm06.

DOI:10.2144/99261bm06
PMID:9894590
Abstract
摘要

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Normalization of RNA hybridization signals by means of SYBR green II-stained 28S or 18S ribosomal RNA and a phosphor imager.
Biotechniques. 1999 Jan;26(1):46-8, 50. doi: 10.2144/99261bm06.
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Unsuitability of using ribosomal RNA as loading control for Northern blot analyses related to the imbalance between messenger and ribosomal RNA content in rat mammary tumors.在大鼠乳腺肿瘤中，由于信使RNA与核糖体RNA含量失衡，使用核糖体RNA作为Northern印迹分析的上样对照不合适。
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[Interaction of mRNA of the p53 protein gene with ribosomal RNA].[p53蛋白基因的信使核糖核酸与核糖体核糖核酸的相互作用]
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Northern blot normalization with a 28S rRNA oligonucleotide probe.使用28S rRNA寡核苷酸探针进行Northern印迹标准化。
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The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase is inappropriate as internal control in comparative studies between skin tissue and cultured skin fibroblasts using Northern blot analysis.在使用Northern印迹分析对皮肤组织和培养的皮肤成纤维细胞进行比较研究时，管家基因甘油醛-3-磷酸脱氢酶不适宜作为内参。
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Expression of distinct maternal and somatic 5.8S, 18S, and 28S rRNA types during zebrafish development.斑马鱼发育过程中不同母源和体细胞5.8S、18S及28S核糖体RNA类型的表达
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