活体猪脑内芬氟拉明作用下区域脑血流量、葡萄糖及氧代谢的定量PET分析

Quantitative PET analysis of regional cerebral blood flow and glucose and oxygen metabolism in response to fenfluramine in living porcine brain.

作者信息

Smith D F, Poulsen P H, Ishizu K, Sakoh M, Hansen S B, Gee A D, Bender D, Gjedde A

机构信息

Institute for Basic Research in Psychiatry, Department of Biological Psychiatry, Aarhus University Psychiatric Hospital, Risskov, Denmark.

出版信息

J Neurosci Methods. 1998 Dec 31;86(1):17-23. doi: 10.1016/s0165-0270(98)00141-1.

DOI:10.1016/s0165-0270(98)00141-1

PMID:9894782

Abstract

The serotonin agonist fenfluramine has been used widely in humans for studying neuronal activation. We carried out the present study in order to determine whether anesthetized pigs could be used for studying effects of fenfluramine on cerebral functions using positron emission tomography (PET). We obtained quantitative measures of regional cerebral blood flow (rCBF) and of glucose and oxygen utilization (rCMRglc and rCMR(O2)) during intravenous administration of fenfluramine, using [15O]water, [18F]FDG and [15O]oxygen, respectively. Fenfluramine (25 mg/h i.v.) caused a significant rise in rCBF and, to a lesser extent, in rCMR2(O2), but it failed to affect rCMRglc. The findings indicate that quantitative estimation of rCBF by repeated injection of [15O]water was more sensitive than either rCMRO2 or rCMRglc for detecting effects of fenfluramine on serotonin neurotransmission in living porcine brain.

摘要

血清素激动剂芬氟拉明已被广泛应用于人体以研究神经元激活。我们开展本研究是为了确定麻醉猪是否可用于使用正电子发射断层扫描（PET）来研究芬氟拉明对脑功能的影响。我们在静脉注射芬氟拉明期间，分别使用[15O]水、[18F]氟代脱氧葡萄糖和[15O]氧，获得了局部脑血流量（rCBF）以及葡萄糖和氧利用量（rCMRglc和rCMR(O2)）的定量测量值。芬氟拉明（静脉注射25毫克/小时）使rCBF显著升高，对rCMR2(O2)的升高幅度较小，但对rCMRglc没有影响。这些发现表明，通过重复注射[15O]水对rCBF进行定量估计，在检测芬氟拉明对活猪脑内血清素神经传递的影响方面，比rCMRO2或rCMRglc更敏感。