复发性自然流产患者的HLA - E多态性

HLA-E polymorphism in patients with recurrent spontaneous abortion.

作者信息

Steffensen R, Christiansen O B, Bennett E P, Jersild C

机构信息

Regional Centre for Blood Transfusion and Clinical Immunology, Aalborg Hospital, Denmark.

出版信息

Tissue Antigens. 1998 Dec;52(6):569-72. doi: 10.1111/j.1399-0039.1998.tb03088.x.

DOI:10.1111/j.1399-0039.1998.tb03088.x

PMID:9894856

Abstract

The aim of this study was to compare the frequencies of five HLA-E alleles in 82 women with recurrent spontaneous abortions with that of 150 random Danish controls. PCR sequence-specific oligonucleotide typing was carried out to detect polymorphism in exons 2 and 3 of the HLA-E gene. In informative samples sequencing of these two exons was also undertaken to confirm the presence of the HLA-E01031 allele. HLA-E0101, HLA-E01032 and HLA-E01031 were detected with frequencies of 56.7%, 33.6% and 9.6% in controls and 58,5%, 32.9% and 8.5% in patients with recurrent abortion, respectively. No HLA-E0102 and E0104 alleles could be detected. Since the HLA-E allele distribution was similar in women with recurrent spontaneous abortion and controls, it is suggested that maternal HLA-E polymorphism per se does not play any role in the pathogenesis of this disorder of pregnancy.

摘要

本研究的目的是比较82例复发性自然流产妇女与150名随机选取的丹麦对照者中5种HLA - E等位基因的频率。采用聚合酶链反应 - 序列特异性寡核苷酸分型法检测HLA - E基因第2和第3外显子的多态性。对于信息充分的样本，还对这两个外显子进行测序以确认HLA - E01031等位基因的存在。在对照者中，HLA - E0101、HLA - E01032和HLA - E01031的检测频率分别为56.7%、33.6%和9.6%，在复发性流产患者中分别为58.5%、32.9%和8.5%。未检测到HLA - E0102和E0104等位基因。由于复发性自然流产妇女与对照者的HLA - E等位基因分布相似，提示母体HLA - E多态性本身在这种妊娠疾病的发病机制中不起任何作用。