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Identification of an HLA-C variant allele, Cw*0805, by sequencing based typing.

作者信息

Dunn P P, Day S, Harvey J, Fuggle S V, Ross J

机构信息

UK Transplant Support Service Authority, Gifford, Bristol.

出版信息

Tissue Antigens. 1998 Dec;52(6):587-9. doi: 10.1111/j.1399-0039.1998.tb03092.x.

DOI:10.1111/j.1399-0039.1998.tb03092.x
PMID:9894860
Abstract

We have identified a variant HLA-C allele, Cw0805, in DNA isolated from a blood sample used in the UK National External Quality Assessment Scheme for Histocompatibility and Immunogenetics. The DNA was initially typed by PCR-SSP and PCR-SBT as Cw0802, 1602 with a single mismatch in exon 2 of one of the alleles. The two HLA-C alleles were separated by locus- and allele-specific PCRs and sequencing analysis showed that the Cw*0802 allele was variant in this sample, with a single mismatch in exon 2 at position 289.

摘要

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