Lee B H, Murugasu-Oei B, Dick T
Institute of Molecular and Cell Biology, Singapore, Republic of Singapore.
Mol Gen Genet. 1998 Dec;260(5):475-9. doi: 10.1007/s004380050919.
The aerobic saprophyte Mycobacterium smegmatis, like its pathogenic counterpart M. tuberculosis, has the ability to adapt to anaerobiosis by shifting down to a dormant state. Here, we report the identification and molecular genetic characterisation of the first dormancy-induced protein in M. smegmatis. Comparative SDS-polyacrylamide gel electrophoresis of protein extracts of aerobically growing and dormant anaerobic M. smegmatis cultures revealed the upregulation of a 27-kDa protein in the dormant state. Peptide sequencing showed that the induced protein is a homologue of the histone-like protein H1p, predicted by the M. tuberculosis genome project. The corresponding hlp gene was cloned from M. smegmatis and sequenced. Disruption of the hlp gene eliminated the histone-like protein but did not affect the viability of the dormant culture.
需氧腐生菌耻垢分枝杆菌，与其致病性对应物结核分枝杆菌一样，具有通过转变为休眠状态来适应厌氧环境的能力。在此，我们报告了耻垢分枝杆菌中首个休眠诱导蛋白的鉴定及分子遗传学特征。对需氧生长和休眠厌氧的耻垢分枝杆菌培养物的蛋白质提取物进行比较SDS聚丙烯酰胺凝胶电泳，结果显示在休眠状态下一种27 kDa蛋白上调。肽测序表明，诱导蛋白是结核分枝杆菌基因组计划预测的类组蛋白H1p的同源物。从耻垢分枝杆菌中克隆并测序了相应的hlp基因。hlp基因的破坏消除了类组蛋白，但不影响休眠培养物的活力。
