Koutsourakis M, Langeveld A, Patient R, Beddington R, Grosveld F
Erasmus University, Medical Genetics Centre, Department of Cell Biology and Genetics, The Netherlands.
Development. 1999 Feb;126(4):723-32.
The gene coding for the murine transcription factor GATA6 was inactivated by insertion of a beta-galactosidase marker gene. The analysis of heterozygote GATA6/lacZ mice shows two inductions of GATA6 expression early in development. It is first expressed at the blastocyst stage in part of the inner cell mass and in the trophectoderm. The second wave of expression is in parietal endoderm (Reichert's membrane) and the mesoderm and endoderm that form the heart and gut. Inactivation leads to a lethality shortly after implantation (5.5 days postcoitum). Chimeric experiments show this to be caused by an indirect effect on the epiblast due to a defect in an extraembryonic tissue.
通过插入β-半乳糖苷酶标记基因，使编码小鼠转录因子GATA6的基因失活。对杂合子GATA6/lacZ小鼠的分析显示，在发育早期GATA6表达有两次诱导。它首先在囊胚期部分内细胞团和滋养外胚层中表达。第二波表达出现在壁内胚层（赖歇特膜）以及形成心脏和肠道的中胚层和内胚层中。失活导致植入后不久（交配后5.5天）出现致死性。嵌合体实验表明，这是由胚外组织缺陷对胚盘外胚层产生的间接影响所致。
