采用三种测定方法分析哺乳诱导的泌乳大鼠腺垂体催乳素浓度变化。

Analysis of suckling-induced changes in adenohypophyseal prolactin concentration in the lactating rat by three assay methods.

作者信息

Nicoll C S, Mena F, Nichols C W, Green S H, Tai M, Russel S M

出版信息

Acta Endocrinol (Copenh). 1976 Nov;83(3):512-21. doi: 10.1530/acta.0.0830512.

DOI:10.1530/acta.0.0830512

Abstract

Suckling-induced changes in adenohypophyseal prolactin (PRL) concentrations in lactating rats were measured in two experiments by three assays: a disc electrophoretic assay (DEA), a bioassay (BA) and a radioimmunoassay (RIA). Substantial discrepancies among the three assays were found in the absolute amounts of pituitary PRL measured and in the changes in adenohypophyseal PRL concentration that were recorded between suckling intervals. The results indicate that in dynamic states of secretory activity the correspondence among BA, RIA and DEA estimates of pituitary PRL concentration is poor.

摘要

在两项实验中，通过三种测定方法测量了哺乳大鼠中吮乳诱导的腺垂体催乳素（PRL）浓度变化：圆盘电泳测定法（DEA）、生物测定法（BA）和放射免疫测定法（RIA）。在测定的垂体PRL绝对量以及在吮乳间隔之间记录的腺垂体PRL浓度变化方面，发现这三种测定方法之间存在显著差异。结果表明，在分泌活动的动态状态下，BA、RIA和DEA对垂体PRL浓度的估计之间的一致性较差。