Crabo B G, Zimmerman K J, Moore R, Thornburgh F H
Biochim Biophys Acta. 1976 Oct 22;444(3):875-85. doi: 10.1016/0304-4165(76)90333-0.
Uptake of 22Na and 42K into ejaculated boar spermatozoa was measured in vitro. Cells were suspended either in seminal plasma or in a biological salt solution of essentially the same composition as boar seminal plasma. Samples were incubated at 30 degrees C. Correction was made for extracellular space in the centrifuged sperm pellet. This was determined as 22Na-space, which was less (P less than 0.001) than [14C] carboxyinulin space. Large differences were observed among individual ejaculates. The half-time for potassium uptake into the spermatozoa averaged 11.5 min, which is much faster than that for leukocytes or erythrocytes. When the spermatozoa were suspended in the biological salt solution, the initial rate of 42K uptake was significantly decreased. This may be due to disturbances of the protein components of the sperm membrane. The uptake of 22Na into the spermatozoa was slow. Sodium and potassium transport appeared not to be coupled in the 3/2 ratio which has been reported for erythrocyte membranes. The average concentration of sodium was 108 mM in seminal plasma and 26 mM in the spermatozoa (112 mmol/kg water and 38 mmol/kg water, respectively). The corresponding figures for potassium were 26 mM and 51 mM (27 mmol/kg water and 74 mmol/kg water). The random error for a single determination for the various methods used varied between 2.4 and 13.3% of the mean.
体外测定了22Na和42K进入射精公猪精子的摄取情况。细胞悬浮于精浆或成分与公猪精浆基本相同的生物盐溶液中。样品在30℃下孵育。对离心后的精子沉淀中的细胞外空间进行了校正。将其确定为22Na空间，该空间小于[14C]羧基菊粉空间（P<0.001）。不同射精之间观察到较大差异。钾进入精子的半衰期平均为11.5分钟，这比白细胞或红细胞的摄取速度快得多。当精子悬浮于生物盐溶液中时，42K的初始摄取速率显著降低。这可能是由于精子膜蛋白质成分受到干扰所致。22Na进入精子的摄取较慢。钠和钾的转运似乎未以红细胞膜报道的3/2比例偶联。精浆中钠的平均浓度为108 mM，精子中为26 mM（分别为112 mmol/kg水和38 mmol/kg水）。钾的相应数值为26 mM和51 mM（分别为27 mmol/kg水和74 mmol/kg水）。所使用的各种方法单次测定的随机误差在平均值的2.4%至13.3%之间。
