Fallani A, Tombaccini D, Ruggieri S
Biochim Biophys Acta. 1976 Nov 19;450(2):210-24. doi: 10.1016/0005-2760(76)90093-x.
In order to study the effect of a dietetic manipulation on the phospholipid molecular structure of a poorly differentiated tumor, the phosphatidylcholines from Yoshida hepatoma cells (AH130) grown either in essential fatty acid deficient or control rats were analyzed comparatively to those from the host livers. Due to essential fatty acid deficiency, the host rat liver exhibited an increased level of mono-unsaturated 1,2-diacyl-sn-glycero-3-phosphocholines, a reduced level of the species contained linoleic acid, and the substitution of tetra- and polyunsaturated-1,2-diacyl-sn-glycero-3-phosphocholines with equivalent amounts of species containing eicosatrienoic acid. The structural analysis of the phosphatidylcholines from Yoshida hepatoma cells grown either in control or essential fatty acid deficient rats revealed the occurrence of 1-alkyl-2-acyl- together with 1,2-diacyl-sn-glycero-3-phosphocholines. The alkyl chains of ether-linked phosphatidylcholines were mainly constituted by 18 : 1, while the acyl chains were characterized by a high level of linoleic and arachidonic or eicosatrienoic acids. The 1,2-diacyl-sn-glycero-3-phosphocholines of the Yoshida hepatoma cells grown in control rats, when compared to those of the liver, showed a higher level of 1,2-disaturated, an increased proportion of mono-unsaturated and a lower proportion of tetra- and polyunsaturated species. In addition, the hepatoma cells showed the occurrence of high proportions of reverse isomeric and random species, such as 1-oleoyl-2-palmitoyl-, 1,2-dioleoyl-, 1-oleoyl-2-linoleoyl- and 1-linoleoyl-2-oleoyl-sn-glycero-3-phosphocholines, scarcely represented in the liver. Growth of Yoshida hepatoma cells in essential fatty acid deficient rats resulted in :(i) the disappearence of 1,2-diacyl-sn-glycero-3-phosphocholines containing linoleic acid; (ii) the substitution of tetra- and and polyunsaturated 1,2-diacyl-sn-glycero-3-phosphocholines with small quantities of species containing eicosatrienoic acid; (iii) an increase of of monounsaturated species, mainly 1-stearoyl-2-oleoyl- and 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholines; (iv) a remarkable increase of 1,2-dioleoyl-sn-1,2-dioleoyl-sn-glycero-3-phosphocholine.
为了研究饮食调控对低分化肿瘤磷脂分子结构的影响，对在必需脂肪酸缺乏或对照大鼠中生长的吉田肝癌细胞（AH130）的磷脂酰胆碱与宿主肝脏的磷脂酰胆碱进行了比较分析。由于必需脂肪酸缺乏，宿主大鼠肝脏中单不饱和1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱水平升高，含亚油酸的种类水平降低，并且等量的含二十碳三烯酸的种类取代了四烯和多不饱和 - 1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱。对在对照或必需脂肪酸缺乏大鼠中生长的吉田肝癌细胞的磷脂酰胆碱进行结构分析，发现存在1 - 烷基 - 2 - 酰基 - 与1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱。醚键连接的磷脂酰胆碱的烷基链主要由18 : 1构成，而酰基链的特征是亚油酸、花生四烯酸或二十碳三烯酸含量高。与肝脏相比，在对照大鼠中生长的吉田肝癌细胞的1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱显示出1,2 - 二饱和水平较高，单不饱和比例增加，四烯和多不饱和种类比例降低。此外，肝癌细胞中出现了高比例的反式异构体和随机种类，如1 - 油酰基 - 2 - 棕榈酰基 - 、1,2 - 二油酰基 - 、1 - 油酰基 - 2 - 亚油酰基 - 和1 - 亚油酰基 - 2 - 油酰基 - sn - 甘油 - 3 - 磷酸胆碱，在肝脏中几乎不存在。吉田肝癌细胞在必需脂肪酸缺乏的大鼠中生长导致：（i）含亚油酸的1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱消失；（ii）四烯和多不饱和1,2 - 二酰基 - sn - 甘油 - 3 - 磷酸胆碱被少量含二十碳三烯酸的种类取代；（iii）单不饱和种类增加，主要是1 - 硬脂酰基 - 2 - 油酰基 - 和1 - 棕榈酰基 - 2 - 棕榈油酰基 - sn - 甘油 - 3 - 磷酸胆碱；（iv）1,2 - 二油酰基 - sn - 甘油 - 3 - 磷酸胆碱显著增加。
