Gali P
Biomedicine. 1976 Sep 30;25(7):268-71.
An electrophoretic study was made of the isozymes of pyruvate kinase (PK) in various adult and foetal tissues, normal or pathological (hypothyroid or dysmature as the result of retardation of intra-uterine growth) rats. The simultaneous kinetic study of PK with and without fructose diphosphate (FDP) showed that retardation of the maturation of the tissues revealed the presence of the M2 isozyme (PK III) longer than the normal foetal age. The increase of negative charges, observed in relation to age, may be the result of structural changes in the enzyme molecule which are yet to be resolved. The variations of relative electrophoretic mobility, and the reactivation of the isozyme by FDP suggest that there is a repression of the synthesis of certain isozymes in normal adult organs.
对正常或病理状态（甲状腺功能减退或因宫内生长迟缓导致发育不全）的成年和胎儿大鼠的各种组织中的丙酮酸激酶（PK）同工酶进行了电泳研究。对有无果糖二磷酸（FDP）时PK的同步动力学研究表明，组织成熟延迟显示M2同工酶（PK III）的存在时间比正常胎儿期更长。观察到的与年龄相关的负电荷增加可能是酶分子结构变化的结果，而这些变化尚待解决。相对电泳迁移率的变化以及FDP对同工酶的再激活表明，正常成年器官中某些同工酶的合成受到抑制。
