Malyshev I I
Biull Eksp Biol Med. 1976 Sep;82(9):1145-6.
The author describes a method used for injury of rabbit fetuses myocardium on the 20-24th days of pregnancy. The abdominal cavity and the uterus of pregnant rats were incised and the amniotic sac and thoracic wall of the fetus -- punctured, thus injuring the heart. Two methods were used: in the first case the amniotic sac was completely drawn out from the uterine cavity, and in the other case -- it remained in the uterus and was punctured through the uterine incision. If the operation was not followed by abortion the animals gave birth to viable full-term rabbits.
作者描述了一种在妊娠第20至24天对兔胎儿心肌造成损伤的方法。切开妊娠大鼠的腹腔和子宫,穿刺胎儿的羊膜囊和胸壁,从而损伤心脏。使用了两种方法:第一种情况是将羊膜囊从子宫腔中完全抽出,另一种情况是——羊膜囊留在子宫内并通过子宫切口进行穿刺。如果手术后没有发生流产,这些动物会产下存活的足月兔。