Baryła-Pankiewicz E
Kliniki Patologii Ciazy, Porodu Instytutu Połoznictwa, Ginekologii Pomorskiej Akademii Medycznej.
Ann Acad Med Stetin. 1992;38:173-89.
The material comprised 11 pregnant rabbits and 95 fetuses stemming from these rabbits. On the 27-th day of pregnancy laparotomy was performed and 1 microgram of thyroxin was administered to the amniotic sac of each fetus in one uterine horn. The fetuses in the second uterine horn, considered as control group, received 0.9% NaCl into their amniotic sac. On the 28-th day cesarean section was carried out, the fluid was collected, the fetuses were taken out, they were weighed and after the lapse of 45 minutes sacrificed by decapitation. The isolated lungs of the fetuses and the amniotic fluid provided the material for further studies. In the amniotic fluid, the content of lecithin and sphingomyelin was determined by thin-layer chromatography. The lungs of fetuses furnished material being used for accomplishing the following types of investigations: determination of mass weight of the wet lungs, and lungs mass after drying, biochemical examination--lecithin and sphingomyelin content in lung homogenates by thin-layer chromatography; histological examination: hematoxylin and eosin stainings by PAS method, argentation by Perdrau method, oil red, toluidine blue stainings as well as electron microscope examination. It has been disclosed that thyroxin administered into the amniotic sac accelerates the fetal lung maturation in rabbits, expressed by higher content of lecithin and by higher ratio of lecithin to sphyngomyelin in fetal lung homogenates at biochemical examinations; it exerts positive effect on the body mass of fetuses and the fetal lung mass, but it fails to influence the lung hydration degree, the sphyngomyelin content in lung homogenates as well as the content of lecithin in the amniotic fluid. The results of histological examination under light microscope have revealed that the lungs of rabbit fetuses in thyroxin group displayed in 70% morphological maturation. The fetal lungs from the control group hardly in 21.1% showed complete maturation. In electron microscopy the fetal lungs in thyroxin group demonstrated more advanced maturation than in the control group, the interalveolar septa were constructed of smaller number of cells, there was a larger number of mature pneumocytes of II type with more abundant lamellar structures, in the alveolar lumen the surfactant appeared markedly more profusely.
材料包括11只怀孕兔子及其95只胎儿。在怀孕第27天进行剖腹手术，向一侧子宫角的每个胎儿羊膜囊中注入1微克甲状腺素。将另一侧子宫角的胎儿作为对照组，向其羊膜囊中注入0.9%氯化钠。在第28天进行剖宫产，收集羊水，取出胎儿，称重，45分钟后断头处死。分离出的胎儿肺和羊水为进一步研究提供了材料。在羊水中，通过薄层色谱法测定卵磷脂和鞘磷脂的含量。胎儿的肺提供了用于进行以下类型研究的材料：测定湿肺的质量重量和干燥后的肺质量，生化检查——通过薄层色谱法测定肺匀浆中卵磷脂和鞘磷脂的含量；组织学检查：苏木精和伊红染色、PAS法染色、佩德劳银染法、油红染色、甲苯胺蓝染色以及电子显微镜检查。结果表明，向羊膜囊中注入甲状腺素可加速兔胎儿肺成熟，生化检查显示胎儿肺匀浆中卵磷脂含量更高，卵磷脂与鞘磷脂的比例更高；它对胎儿体重和胎儿肺重量有积极影响，但对肺水合程度、肺匀浆中鞘磷脂含量以及羊水中卵磷脂含量没有影响。光学显微镜下的组织学检查结果显示，甲状腺素组兔胎儿肺有70%呈现形态成熟。对照组胎儿肺仅有21.1%显示完全成熟。电子显微镜检查显示，甲状腺素组胎儿肺的成熟程度高于对照组，肺泡间隔由较少的细胞构成，有更多数量的成熟II型肺细胞，其板层结构更丰富，肺泡腔内表面活性物质明显更丰富。
