Patel O V, Camous S, Takenouchi N, Takahashi T, Hirako M, Sasaki N, Domeki I
Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka.
Res Vet Sci. 1998 Nov-Dec;65(3):195-9. doi: 10.1016/s0034-5288(98)90142-4.
This study characterised the peripheral plasma concentration of PSP-60 throughout gestation, and examined the effect of stage of gestation and foetal number on this protein in Holstein cows after non-surgical embryo transfer. Cows (n=12) were divided into two groups; Group 1 contained single embryo recipient cows (n=5), Group 2 contained twin-embryo recipient cows (n=7). Blood was collected approximately every third day from day 0 (first day of standing oestrus), then daily for the last 10 days of gestation and until one day post-partum. Two of the twin-embryo recipient cows had abnormal pregnancies, consequently data from them was considered separately. In both groups PSP-60 increased progressively from about day 20 post-oestrus to 20 days pre-partum (from 0.9 +/- 0.2 to 49.7 +/- 8.7 ng ml(-1), and from 1.3 +/- 0.6 to 115 +/- 34.9 ng ml(-1) (mean +/- SEM), in singleton and twin-bearing groups, respectively). The mean concentrations between 20 and 10 days pre-partum increased dramatically by about six-fold (P<0.001) in singleton-bearing cows (from 49.7 +/- 8.7 ng ml(-1) to 283.8 +/- 73.7 ng ml(-1)) to over two-fold in twin-bearing cows (from 115 +/- 34.9 ng ml(-1) to 284 +/- 98.2 ng ml(-1)). The mean concentrations of the two groups were indistinguishable between 10 days pre-partum and parturition. Cows giving birth prematurely to stillborn calves or to a schistosomus reflexus calf exhibited abnormal PSP-60 profiles. Our findings indicate that peripheral plasma PSP-60 concentrations are correlated to the stage of gestation and foetal number, and assist in predicting foeto-placental viability.
本研究对整个妊娠期荷斯坦奶牛外周血浆中PSP - 60的浓度进行了表征，并研究了非手术胚胎移植后妊娠阶段和胎儿数量对该蛋白的影响。将奶牛（n = 12）分为两组；第1组包含单胚胎受体奶牛（n = 5），第2组包含双胚胎受体奶牛（n = 7）。从第0天（发情站立的第一天）开始大约每三天采集一次血液，然后在妊娠的最后10天每天采集，直至产后一天。两名双胚胎受体奶牛出现异常妊娠，因此对它们的数据进行单独分析。在两组中，PSP - 60从发情后约第20天到产前20天逐渐增加（单胎组从0.9±0.2增加到49.7±8.7 ng/ml，双胎组从1.3±0.6增加到115±34.9 ng/ml（平均值±标准误））。在产前20天到产前10天之间，单胎奶牛的平均浓度显著增加约6倍（P < 0.001）（从49.7±8.7 ng/ml增加到283.8±73.7 ng/ml），双胎奶牛增加超过2倍（从115±34.9 ng/ml增加到284±98.2 ng/ml）。两组在产前10天和分娩之间的平均浓度没有差异。早产死胎或脊柱裂胎儿的奶牛表现出异常的PSP - 60谱。我们的研究结果表明，外周血浆PSP - 60浓度与妊娠阶段和胎儿数量相关，并有助于预测胎儿 - 胎盘的活力。
