一种通过亲和层析分离得到的输卵管酶，它作用于阿根廷蟾蜍体腔卵母细胞的卵黄膜。
An oviducal enzyme isolated by affinity chromatography which acts upon the vitelline envelope of Bufo arenarum coelomic oocytes.
作者信息
Miceli D C, Fernández S N, del Pino E J
出版信息
Biochim Biophys Acta. 1978 Sep 11;526(1):289-92. doi: 10.1016/0005-2744(78)90313-3.
PMID:99176
Abstract
A trypsin-like oviducal proteinase acting upon the vitelline envelope of Bufo arenarum coelomic oocytes has been purified to apparent homogeneity by gel filtration on Sephadex G-200 and by affinity chromatography on a column of Sepharose 4-B containing covalently bound concanavlin A (Con A). The biologically active molecule migrated as a single band of protein upon SDS polyacrylamide gel electrophoresis.
摘要
一种作用于南美蟾蜍体腔卵母细胞卵黄膜的类胰蛋白酶输卵管蛋白酶，通过在Sephadex G - 200上进行凝胶过滤以及在含有共价结合伴刀豆球蛋白A（Con A）的Sepharose 4 - B柱上进行亲和层析，已被纯化至表观均一性。该生物活性分子在SDS聚丙烯酰胺凝胶电泳中迁移为单一蛋白质条带。
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