Galmés A, Besalduch J, Bargay J, Novo A, Morey M, Guerra J M, Duran M A
Servicio de Hematologia y Hemoterapia, Hospital Universitario Son Dureta, Palma De Mallorca, Spain.
Transfusion. 1999 Jan;39(1):70-3. doi: 10.1046/j.1537-2995.1999.39199116897.x.
Hematopoietic progenitor cells (HPCs) can be cryopreserved and stored below -120 degrees C in liquid nitrogen or at -80 degrees C in mechanical freezers.
The feasibility of long-term storage of HPCs at -80 degrees C was investigated. The studies included a comparison of 5- and 10-percent dimethyl sulfoxide (DMSO) as cryoprotectant at various lengths of storage time. Mononuclear cell (MNC) recovery and viability and colony-forming unit-granulocyte-macrophage (CFU-GM) and burst-forming unit-erythroid (BFU-E) recovery assays were performed. The peripheral blood HPCs of 24 consecutive patients included in the program of autologous transplantation were studied.
The MNC viability decreased progressively with the length of time from cryopreservation, reaching 32 percent after 31 months of storage. The recovery rates of CFU-GM and BFU-E also decreased progressively with the duration of frozen storage, to 50 and 43.5 percent, respectively, after 12 months and to 0 percent (both) after 24 months. At 6 months of storage, MNC viability was 80 percent, and CFU-GM and BFU-E recovery was 63.5 and 80.5 percent, respectively. There were no differences between MNCs cryopreserved with 5- or 10-percent DMSO in terms of cell viability. There were no differences between CFU-GM recovery or BFU-E recovery from HPCs cryopreserved in 5- or 10-percent DMSO. Patients given HPCs stored in these conditions for periods ranging between 123 and 202 days showed a complete and rapid hematologic recovery.
HPCs can be cryopreserved at -80 degrees C with 5-percent DMSO and stored at -80 degrees C no longer than 6 months. A 5-percent DMSO concentration is comparable to a with 10-percent concentration in terms of recovery and MNC viability.
造血祖细胞（HPCs）可被冷冻保存，并在液氮中于-120℃以下或在机械冷冻箱中于-80℃储存。
研究了在-80℃长期储存HPCs的可行性。研究包括比较5%和10%二甲基亚砜（DMSO）作为冷冻保护剂在不同储存时间的效果。进行了单核细胞（MNC）回收率和活力以及集落形成单位-粒细胞-巨噬细胞（CFU-GM）和爆式红系集落形成单位（BFU-E）回收率测定。对纳入自体移植项目的24例连续患者的外周血HPCs进行了研究。
MNC活力从冷冻保存后随时间逐渐下降，储存31个月后降至32%。CFU-GM和BFU-E的回收率也随冷冻储存时间逐渐下降，12个月后分别降至50%和43.5%，24个月后均降至0%。储存6个月时，MNC活力为80%，CFU-GM和BFU-E回收率分别为63.5%和80.5%。用5%或10%DMSO冷冻保存的MNC在细胞活力方面无差异。用5%或10%DMSO冷冻保存的HPCs在CFU-GM回收率或BFU-E回收率方面无差异。接受在这些条件下储存123至202天的HPCs的患者显示出完全且快速的血液学恢复。
HPCs可用5%DMSO在-80℃冷冻保存，并在-80℃储存不超过6个月。就回收率和MNC活力而言，5%DMSO浓度与10%浓度相当。
