Liu M C, Sakakibara Y, Liu C C
Department of Biochemistry, University of Texas Health Center, Tyler, Texas, 75708,
Biochem Biophys Res Commun. 1999 Jan 8;254(1):65-9. doi: 10.1006/bbrc.1998.9872.
Analysis of the nucleotide sequence of a recently cloned mouse sulfotransferase cDNA (clone 679153) revealed the presence in its 3'-untranslated sequence of an AT-rich region which contains four ATTTA motifs and an TTATTTAT-like sequence, commonly found among those encoding inflammation-related proteins. The recombinant enzyme expressed in Escherichia coli and purified to near electrophoretic homogeneity displayed strong sulfotransferase activities toward various prostaglandins, thromboxane B2, and leukotriene E4. These results mark the first discovery of the sulfation of eicosanoids catalyzed by a distinct sulfotransferase.
对最近克隆的小鼠磺基转移酶cDNA（克隆679153）的核苷酸序列分析显示，在其3'-非翻译序列中存在一个富含AT的区域，该区域包含四个ATTTA基序和一个TTATTTAT样序列，这些序列在编码炎症相关蛋白的序列中很常见。在大肠杆菌中表达并纯化至近乎电泳纯的重组酶对各种前列腺素、血栓素B2和白三烯E4表现出很强的磺基转移酶活性。这些结果标志着首次发现由一种独特的磺基转移酶催化的类二十烷酸硫酸化作用。
