Hauser H, Dyer J H, Nandy A, Vega M A, Werder M, Bieliauskaite E, Weber F E, Compassi S, Gemperli A, Boffelli D, Wehrli E, Schulthess G, Phillips M C
Institute of Biochemistry, Swiss Federal Institute of Technology, ETH Centre, Zurich.
Biochemistry. 1998 Dec 22;37(51):17843-50. doi: 10.1021/bi982404y.
Here we show that scavenger receptor class B type I is present in the small-intestine brush border membrane where it facilitates the uptake of dietary cholesterol from either bile salt micelles or phospholipid vesicles. This receptor can also function as a port for several additional classes of lipids, including cholesteryl esters, triacylglycerols, and phospholipids. It is the first receptor demonstrated to be involved in the absorption of dietary lipids in the intestine. In liver and steroidogenic tissues, the physiological ligand of this receptor is high-density lipoprotein. We show that binding of high-density lipoprotein and apolipoprotein A-I to the brush border membrane-resident receptor inhibits uptake of cholesterol (sterol) into the brush border membrane from lipid donor particles. This finding lends further support to the conclusion that scavenger receptor BI catalyzes intestinal cholesterol uptake. Our findings suggest new therapeutic approaches for limiting the absorption of dietary cholesterol and reducing hypercholesterolemia and the risk of atherosclerosis.
我们在此表明，I型B类清道夫受体存在于小肠刷状缘膜中，它有助于从胆盐微团或磷脂囊泡中摄取膳食胆固醇。该受体还可作为包括胆固醇酯、三酰甘油和磷脂在内的几类其他脂质的转运通道。它是首个被证实参与肠道膳食脂质吸收的受体。在肝脏和类固醇生成组织中，该受体的生理配体是高密度脂蛋白。我们发现，高密度脂蛋白和载脂蛋白A-I与刷状缘膜驻留受体的结合会抑制胆固醇（甾醇）从脂质供体颗粒进入刷状缘膜。这一发现进一步支持了清道夫受体BI催化肠道胆固醇摄取的结论。我们的研究结果提示了限制膳食胆固醇吸收、降低高胆固醇血症和动脉粥样硬化风险的新治疗方法。
