大肠杆菌的udhA基因编码一种可溶性吡啶核苷酸转氢酶。
The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase.
作者信息
Boonstra B, French C E, Wainwright I, Bruce N C
机构信息
Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom.
出版信息
J Bacteriol. 1999 Feb;181(3):1030-4. doi: 10.1128/JB.181.3.1030-1034.1999.
Abstract
The udhA gene of Escherichia coli was cloned and expressed in E. coli and found to encode an enzyme with soluble pyridine nucleotide transhydrogenase activity. The N-terminal end of the enzyme contains the fingerprint motif of a dinucleotide binding domain, not present in published E. coli genome sequences due to a sequencing error. E. coli is hereby the first organism reported to possess both a soluble and a membrane-bound pyridine nucleotide transhydrogenase.
摘要
大肠杆菌的udhA基因被克隆并在大肠杆菌中表达，发现其编码一种具有可溶性吡啶核苷酸转氢酶活性的酶。该酶的N末端含有二核苷酸结合结构域的指纹基序，由于测序错误，已发表的大肠杆菌基因组序列中不存在该基序。因此，大肠杆菌是首个被报道同时拥有可溶性和膜结合吡啶核苷酸转氢酶的生物体。
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本文引用的文献
1
Activation of the OxyR transcription factor by reversible disulfide bond formation.通过可逆二硫键形成激活OxyR转录因子。
Science. 1998 Mar 13;279(5357):1718-21. doi: 10.1126/science.279.5357.1718.
2
Transcriptional regulation of the Escherichia coli oxyR gene as a function of cell growth.大肠杆菌oxyR基因的转录调控与细胞生长的关系
J Bacteriol. 1997 Oct;179(19):6181-6. doi: 10.1128/jb.179.19.6181-6186.1997.
3
Cloning, sequence, and properties of the soluble pyridine nucleotide transhydrogenase of Pseudomonas fluorescens.荧光假单胞菌可溶性吡啶核苷酸转氢酶的克隆、序列及特性
J Bacteriol. 1997 Apr;179(8):2761-5. doi: 10.1128/jb.179.8.2761-2765.1997.
4
The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse.
Biochim Biophys Acta. 1996 Mar 28;1273(3):203-5. doi: 10.1016/0005-2728(95)00159-x.
5
Proton-translocating transhydrogenase in bacteria.
Biochem Soc Trans. 1993 Nov;21(4):1010-3. doi: 10.1042/bst0211010.
6
Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection.OxyR与DNA的结合沿扩展的DNA结合位点发生氧化还原依赖性移位：一种差异启动子选择机制。
Cell. 1994 Sep 9;78(5):897-909. doi: 10.1016/s0092-8674(94)90702-1.
7
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Microbiology (Reading). 1994 Jul;140 ( Pt 7):1595-604. doi: 10.1099/13500872-140-7-1595.
8
Pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Improved purification, physical properties and subunit arrangement in purified polymers.
Eur J Biochem. 1980 Oct;111(2):347-55. doi: 10.1111/j.1432-1033.1980.tb04948.x.
9
Why are two different types of pyridine nucleotide transhydrogenase found in living organisms?为什么在生物体内会发现两种不同类型的吡啶核苷酸转氢酶？
Eur J Biochem. 1983 Apr 5;131(3):527-33. doi: 10.1111/j.1432-1033.1983.tb07293.x.
10
Purification and properties of the pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa.铜绿假单胞菌吡啶核苷酸转氢酶的纯化及性质
J Biol Chem. 1970 Jun 10;245(11):2825-36.
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