在毕赤酵母中表达的重组人淀粉样前体样蛋白2（APLP2）可刺激神经突生长。
Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth.
作者信息
Cappai R, Mok S S, Galatis D, Tucker D F, Henry A, Beyreuther K, Small D H, Masters C L
机构信息
Department of Pathology, The University of Melbourne and The Mental Health Research Institute of Victoria, Parkville, Australia.
出版信息
FEBS Lett. 1999 Jan 8;442(1):95-8. doi: 10.1016/s0014-5793(98)01635-4.
The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of the APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins.
人类淀粉样前体样蛋白2（APLP2）是阿尔茨海默病淀粉样前体蛋白（APP）基因家族的成员。人APLP2胞外结构域（sAPLP2）在毕赤酵母中表达，重组sAPLP2通过金属螯合琼脂糖层析从培养基中一步纯化得到。在鸡交感神经元上，将APLP2的神经营养活性与APP亚型sAPP695和sAPP751进行了比较。APLP2具有与APP亚型相似的促进神经突生长的活性。这表明APP和APLP2具有相似或相关的作用，并支持APP基因家族蛋白之间功能冗余的观点。
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