Chirkov G P, Sizova S T, Efremova O I, Gutnikova M N, Kudriashova E A
Genetika. 1976;12(6):95-102.
A number of methods which greatly simplify the introduction of frame shift mutations in the limited segment of the phage T4 rII genes were developed for studying genetical recombination between closely linked markers. These methods enable to construct multiple rII mutants with relatively small expenditure of labour. Suppression of gene 30 deficiency by rII mutations has been studied for a variety of conditions. The results obtained indicate that rIIB polypeptide is involved in two different activities: rIIB region which is dispensable for growth on lambda-lysogenic Escherichia coli strains behaves as indispensable in phenomenon of suppression of ligase deficiency.
为了研究紧密连锁标记之间的基因重组，已开发出多种方法，这些方法极大地简化了在噬菌体T4 rII基因有限片段中引入移码突变的过程。这些方法能够以相对较少的工作量构建多个rII突变体。已在多种条件下研究了rII突变对基因30缺陷的抑制作用。所得结果表明，rIIB多肽参与两种不同的活性：在λ-溶原性大肠杆菌菌株上生长时可缺失的rIIB区域，在连接酶缺陷抑制现象中表现为不可或缺。
