Neuhaus M, Wagner U, Schmid U, Ackermann D, Zellweger T, Maurer R, Alund G, Knönagel H, Rist M, Moch H, Mihatsch M J, Gasser T C, Sauter G
Institute for Pathology and Urologic Clinics, University of Basel, Switzerland.
Hum Pathol. 1999 Jan;30(1):81-6. doi: 10.1016/s0046-8177(99)90305-2.
A disturbed cellular DNA content is of potential diagnostic and prognostic relevance in urinary bladder cancer. To evaluate the prognostic significance of individual chromosomal aberrations in superficial bladder cancer, specimens of 105 tumors (67 pTa, 38 pT1) were examined by fluorescence in situ hybridization (FISH). FISH allows quantitation of chromosomes on a cell by cell level. Centromere probes for the chromosomes Y, 1, and 17 were used. There was a strong association between polysomies of the chromosomes 1 (found in 46% of tumors) and 17 (40% of tumors, P < .0001). Polysomies (1 and 17) were significantly more frequent in pT1 than in pTa tumors (P < .0001 each). In pTa tumors, polysomies of both chromosomes were linked to a high risk of recurrences; polysomy 17 was associated with an increased risk of progression (P < .05 each). There was no significant association between polysomies and an unfavorable prognosis in pT1 carcinomas. Previous studies had suggested a prognostic role of Y losses in bladder cancer. However, Y losses were not linked to recurrences or tumor progression in pTa or pT1 tumors of 67 male patients. These data show that marked genetic differences exist between pTa and pT1 carcinomas. They also indicate that polysomies of different chromosomes may have prognostic relevance in pTa urinary bladder cancer.
细胞DNA含量紊乱在膀胱癌中具有潜在的诊断和预后意义。为了评估浅表性膀胱癌中单个染色体畸变的预后意义，采用荧光原位杂交（FISH）技术检测了105例肿瘤标本（67例pTa，38例pT1）。FISH技术能够在细胞水平上对染色体进行定量分析。使用了Y、1和17号染色体的着丝粒探针。1号染色体（46%的肿瘤中发现）和17号染色体（40%的肿瘤中发现，P < 0.0001）的多倍体之间存在强关联。1号和17号染色体的多倍体在pT1肿瘤中比在pTa肿瘤中显著更常见（均为P < 0.0001）。在pTa肿瘤中，两条染色体的多倍体都与高复发风险相关；17号染色体多倍体与进展风险增加相关（均为P < 0.05）。在pT1癌中，多倍体与不良预后之间无显著关联。先前的研究曾提示Y染色体缺失在膀胱癌中的预后作用。然而，在67例男性患者的pTa或pT1肿瘤中，Y染色体缺失与复发或肿瘤进展无关。这些数据表明pTa和pT1癌之间存在明显的遗传差异。它们还表明不同染色体的多倍体在pTa膀胱癌中可能具有预后意义。
