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鸡胚原代视网膜细胞中的烯醇化酶活性不受暴露于60赫兹电场的影响。

Enolase activity in chicken embryo primary retina cells is not affected by exposure to a 60-Hz electric field.

作者信息

Dutta S K, Nazar A S, Verma M

机构信息

Department of Biology and Cancer Research Center, Howard University, Washington, DC 20059, USA.

出版信息

Cancer Biochem Biophys. 1998 Jun;16(1-2):45-52.

PMID:9923966
Abstract

This study was to determine the extent of alteration of enolase specific activities in chicken embryo retina primary cells in culture when exposed to an ELF (extremely low frequency) electric field at 60 Hz. Results showed no alteration of enolase activity and enolase mRNA levels. In this study, sham vs. control experiments were also conducted to neutralize ambient AC magnetic fields, stray magnetic fields and variations in field uniformity. Under similar conditions, the specific activity of enolase is decreased in neuroblastoma cell line (NG108). It is apparent from this study that primary cells either are not affected by these exposure conditions or the effect is transient and warrants no damage.

摘要

本研究旨在确定鸡胚视网膜原代细胞在体外培养时,暴露于60Hz极低频(ELF)电场下烯醇化酶比活性的改变程度。结果显示烯醇化酶活性和烯醇化酶mRNA水平没有改变。在本研究中,还进行了假对照与对照实验,以抵消环境交变磁场、杂散磁场和场均匀性变化的影响。在相似条件下,神经母细胞瘤细胞系(NG108)中烯醇化酶的比活性降低。从本研究可以明显看出,原代细胞要么不受这些暴露条件的影响,要么这种影响是短暂的,不会造成损伤。

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