Sukemori S, Sugimura K
Tokyo University of Agriculture, Department of Zootechnical Science, Japan.
Cancer Biochem Biophys. 1998 Jun;16(1-2):53-61.
Cultured P388 (murine) and CEM (human) leukemia cells were exposed to medium including either 5-fluorouracil (5-FU) or methotorexate (MTX). The level of drug was less than the ID50 value obtained in RPMI 1640 medium (control). Enhancement of drug cytotoxicity was determined with medium in which asparagine or glutamine level had been reduced to 60% of the level of the control. Proliferation of both types of cells for 3 days showed the cytotoxicities of the drugs. Asparagine reduced medium showed no enhancement of cytotoxicity in comparison with control, while glutamine reduced medium enhanced the cytotoxicity of 5-FU, but not that of MTX. Regulation of extracellular glutamine level seemed to affect stage G1 of the cell cycle, as found in the previous result with adriamycin.
培养的P388（鼠源）和CEM（人源）白血病细胞暴露于含有5-氟尿嘧啶（5-FU）或甲氨蝶呤（MTX）的培养基中。药物浓度低于在RPMI 1640培养基（对照）中获得的ID50值。用天冬酰胺或谷氨酰胺水平降低至对照水平60%的培养基来测定药物细胞毒性的增强情况。两种细胞3天的增殖情况显示了药物的细胞毒性。与对照相比，天冬酰胺降低的培养基未显示细胞毒性增强，而谷氨酰胺降低的培养基增强了5-FU的细胞毒性，但未增强MTX的细胞毒性。如先前用阿霉素得到的结果一样，细胞外谷氨酰胺水平的调节似乎影响细胞周期的G1期。
